HTAS Application

Bars with (*) may require high speed access.

HTAS Application: Absorption Studies

Materials:

Oral Absorption - Intestinal tissues were used in whole or its mucosa stripped from underlying layers. Artificial “functionalized” membranes with or without attached cells or microfilters impregnated with phospholipid solution may be used as well.

Absorption studies may be conducted also on buccal, nasal, corneal, vaginal, skin and lung tissues.

Procedure:

A compound under study is injected into the supply chamber facing the mucosal surface of the epithelial tissue or into the supply buffer reservoir. Another buffer is injected into the receiving chamber facing the serosal surface of the tissue. The mucosal to serosal flux represents compound absorption. For serosal to mucosal flux studies, the compound is injected into the receiving chamber and its concentration in the supplying chamber is investigated.

To measure compound absorption, samples of compound/buffer solutions can be taken from the receiving chamber or both chambers at the end or during the incubation cycle. Sampling of compounds is interfaced with analytical instrumentation (SP, GC, MS etc.). Compound accumulation in tissue can be evaluated after tissue removal and analysis.

Analysis:

The HTAS cartridge assembly enables the study of compound/tissue fluxes from supplying chamber to receiving chamber or vice versa and may be used for the analysis of:

	Compound transport through the membrane that may characterize absorption;
	Compound accumulation in the tissue;
	Transport through apical and basolateral membrane separately;
	First-pass metabolism;
	P-glycoprotein activity (cell excretion systems);
	Transmembrane potential difference and passing current.
	Mechanisms of compound permeation and absorption;
	Effect of inhibitors and enhancers of permeation and absorption;
	Pharmacologic antagonism in toxicity studies;
	Various compound (nutrients, drugs, cosmetics, other xenobiotics) interactions.

Please contact us for more detailed information.